The Properties of Enzymes Lab Report - Biology - Stuvia

 

properties of enzymes lab report

Enzymes Experiment Introduction Enzymes are biological catalysts that promote some of the thousands of chemical reactions that occur in living cells. Lab report introduction on enzymes. Lab 3 Enzymes. In this lab session, we'll measure the reaction rate of the catalyzed reaction of hydrogen peroxide by adding a fixed amount of catalase enzyme. Chapter 2: Properties of Enzymes Purpose: The purpose of the chapter 2 lab was to determine a variety of enzymatic properties. Enzymes are large proteins which act as catalysts in many biological reactions. An enzyme is composed of one or more polypeptide chains, which are uniquely folded into an elaborate 3 dimensional shape. One portion of the protein is folded in a specific way in order to %(16). Unformatted text preview: Elizabeth van Dijk 02 18 14 Bio B Properties of Enzymes and the Effects of the Metal Copper Sulfate CuSO on Catalase Abstract In this lab we explored the properties and role of enzymes in biological systems This was accomplished by observing the addition of hydrogen peroxide to water a piece of potato and the enzyme catalase Also we observed and recorded data.


Liver Enzyme Lab Report


AP Biology, Mod 5. The purpose of this lab was to test enzyme function in various environments. Substrate concentration, properties of enzymes lab report, temperature, and pH all affect the chemical reaction. In this lab, the enzyme catalase was used to break down hydrogen peroxide into less toxic water and oxygen gas.

Using quantitative as well as qualitative observation the concept that enzymes remain after a reaction was confirmed from the first test. After testing liver, apple, and potato it was concluded that liver contained the most catalase.

The final test focused on the reaction rate of liver in varying pH solutions. The relationship between catalase reaction rate and pH was shown to be parabolic with a peak near neutral. This lab covered the measuring of the effects of changes in temperature, pH, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment.

The questions of how environmental factors affect the rate of enzyme-catalyzed reactions were answered in this lab. The reaction rates of enzymes were very much affected by changes in temperature, pH and enzyme concentration. The enzyme studied in this lab was catalase.

Catalase breaks down hydrogen peroxide, which is toxic, into 2 safe substances- water and oxygen, by speeding up a reaction, properties of enzymes lab report. Enzymes such as catalase are vital to our body, because if toxic substances such hydrogen peroxide were not broken down into harmless substances in our body, then they would poison our cells. When the substrate of hydrogen peroxide was added to the substances each performed a vastly different reaction. After dropping the Hydrogen Peroxide on the apple samples there was no reaction indicated therefore receiving a rating of 0.

Once the same amount of the substrate was added to the potato there was a slight fizzy and bubbly reaction thus earning the rating of 2. The liver catalase was then tested and resulted in the largest reaction that was given a rating of 5 due to the speed and magnitude.

After testing properties of enzymes lab report substance the group then began to look into optimal conditions of pH for the reactions. After adding Hydrochloric Acid onto the liver the reaction rate seemed to diminish as the acidity increased. Catalase: Liver, properties of enzymes lab report. Substrate: Hydrogen Peroxide. Looking at our data for the first experiment, we can infer that the liver contains the catalase enzyme while the hydrogen peroxide is the substrate.

This is true because in the reaction, the hydrogen peroxide is observed to have gone through a chemical change; turning into water molecules and dioxide molecules, whereas the liver stayed the same.

Because the substrate already went through the chemical reaction, properties of enzymes lab report could not go through another one with new catalase, but the old catalase still had functional enzymes and could therefore be reused.

In the second part of the experiment, the intensity of the reaction in ascending order was apple, potato, and liver. The apple sample is simply carbohydrates meant as food for the apple seed and will have no enzymes in it, thus no reaction was observed. The potato will have a semi-intense reaction because only a small concentration of the sample of potato is enzymes whereas the rest is starch and organic compounds.

The liver will have a large reaction because the majority of liver is proteins and enzymes which break down the Hydrogen Peroxide. In the third experiment, it was inferred that as the acidity increased in the substrate, the rate of reaction decreased.

Our data shows a linear trend, however, research shows that the trend is in fact a parabola opening downwards with a maximum at a pH of 5, the acidity of Hydrogen Peroxide alone.

Because we were only able to stimulate an environment of a lower pH, we were only able to get 1 direction of slope, showing linear results. This shows that enzymes only work in a certain pH level. Through our experimentation the reaction between the enzyme catalase and hydrogen peroxide was tested. Repeating properties of enzymes lab report reaction with various materials allowed for the concentration of catalase to be inferred.

Using liver as our control, we changed properties of enzymes lab report pH of the solution in each trial to test the reaction rate as the properties of enzymes lab report increased. We concluded that as the pH of a solution increased the reaction rate increased inversely until a pH of about 5. The error within our experiment only altered the specific pH but the overall result of the last test remains true.

Works Cited What are Enzymes? Retrieved October 14, Biology Lab Notebook. Search this site. Chick Development Lab. Coli Transformation Lab. Fermentation Lab, properties of enzymes lab report.

Lab 5 - Mitosis. Lab 7 - Gel Electrophoresis, properties of enzymes lab report. Lab Report 1 - Osmosis. Lab Report 2 - Organic Chemistry. Lab Report 3 - Coacervates. Lab Report 4 - Enzymes. Lab Report 6 - Hardy-Weinberg. DNA Think Quest.

Geologic Time. Homeostasis and Cell Transport. Human Traits. Wootton on October, 8 Throughout the three parts of the lab, the ability for enzymes to break down was studied.

Once this was done, we took the leftover liquids and used it on a new piece of meat to see if the enzyme was reusable. In Part B, we tested, observed, and recorded the rate of reaction with Hydrogen Peroxide on three substances: apple, liver, properties of enzymes lab report, and potato.

In Part C, we altered the pH of the solution by adding drops of diluted Hydrochloric Acid to see if it had an affect on the rate of reaction in catalyses on pieces of liver.

 

Properties of Enzymes Lab Report - BIO - NCSU - GradeBuddy

 

properties of enzymes lab report

 

Enzymes contain a cavity called the active site, to which the substrate, the substance whose reaction is catalyzed by the enzyme, bonds, forming the enzyme-substrate complex. Only one type of substrate can bond with the enzyme, in a lock-and-key manner. The enzyme then breaks the substrate down to form the products of the organic reaction. The Properties of Enzymes Lab Report This lab report examines the different properties that enzymes exhibit. If you take the "Biology 1" college course, this lab report will most likely be used. Enzymes. The Rate of Reaction on Liver, Apples, and Potatoes with Hydrogen Peroxide. AP Biology, Mod 5. Abstract The purpose of this lab was to test enzyme function in various environments. Substrate concentration, temperature, and pH all affect the chemical reaction.